This detailed overview may help your with your decision regarding which dyes to choose for your individual experiment.
--> Alexa Fluor 350 [Alexa350] --> DY-415 [DY415] --> ATTO 425 [ATTO425] --> ATTO 465 [ATTO465] --> Bodipy FL [BOFL] --> Alexa Fluor 488 [Alexa488] --> FAM [FAM] --> Fluorescein isothiocyanate [FITC] --> Fluoresceine [FLU] --> Fluorescein-dT [FLUdT] --> ATTO 488 [ATTO488] --> Oregon Green 488 [OG488] --> Oregon Green 514 [OG514] --> Rhodamine Green [RGR] --> TET [TET] --> ATTO 520 [ATTO520] --> JOE [JOE] --> Yakima Yellow [YAKYE] --> Bodipy 530/550 [BO530] --> ATTO 532 [ATTO532] --> HEX [HEX] --> Alexa Fluor 555 [Alexa555] --> Bodipy TMR-X [BOTMRX] --> Cyanine3 [CY3] --> ATTO 550 [ATTO550] --> TAMRA [TAM] --> Rhodamine Red [RRE] --> ATTO 565 [ATTO565] --> ATTO RHO12 [ATTORHO12] --> ROX [ROX] --> Texas Red [TxRed] --> Cyanine3.5 [CY35] --> LightCycler 610 [LC610] --> ATTO 594 [ATTO594] --> DY-480 XL [DY480] --> DY-610 [DY610] --> ATTO 610 [ATTO610] --> LightCycler 640 [LC640] --> Bodipy 630/650 [BO630] --> ATTO 633 [ATTO633] --> Alexa Fluor 647 [Alexa647] --> Bodipy 650/665 [BO650] --> ATTO 647N [ATTO647N] --> Cyanine5 [CY5] --> DY-649 [DY649] --> Cyanine5.5 [CY55] --> ATTO 680 [ATTO680] --> DY-682 [DY682] --> ATTO 700 [ATTO700] --> ATTO 740 [ATTO740] --> DY-782 [DY782]Dye | Abs | Em | Ext. coeff. | Mol. weight | 5' | Int | 3' |
Alexa Fluor 350 [Alexa350] | 346 | 442 | 19,000 | 409.35 | x | x | x |
Dy-415 [DY415] | 418 | 467 | 34,000 | 552 | x | x | x |
ATTO 425 [ATTO425] | 436 | 484 | 45,000 | 499 | x | x | x |
ATTO 465 [ATTO465] | 453 | 508 | 75,000 | 393 | x | x | x |
Bodipy FL [BOFL] | 504 | 510 | 70,000 | 391.3 | x | x | x |
Alexa Fluor 488 [Alexa488] | 495 | 519 | 71,000 | 630 | x | x | x |
FAM [FAM] | 495 | 520 | 83,000 | 474.5 | x | - | - |
Fluorescein isothiocyanate [FITC] | 495 | 520 | 73,000 | 505 | x | x | x |
Fluorescein [FLU] | 495 | 520 | 83,000 | 504 | - | - | x |
Fluorescein-dT [FLUdT] | 494 | 522 | 75,000 | 472.71 | - | x | - |
ATTO 488 [ATTO488] | 501 | 523 | 90,000 | 687 | x | x | x |
Oregon Green 488 [OG488] | 496 | 524 | 76,000 | 510 | x | x | x |
Oregon Green 514 [OG514] | 506 | 526 | 85,000 | 609.4 | x | x | x |
Rhodamine Green [RGR] | 503 | 528 | 74,000 | 472 | x | x | x |
TET [TET] | 521 | 536 | 73,000 | 612.3 | x | - | - |
ATTO 520 [ATTO520] | 516 | 538 | 1,10,000 | 464 | x | x | x |
JOE [JOE] | 520 | 548 | 73,000 | 603.4 | x | x | x |
Yakima Yellow [YAKYE] | 530 | 549 | 84,000 | 654.3 | x | - | - |
Bodipy 530/550 [BO530] | 534 | 551 | 77,000 | 514.31 | x | x | x |
HEX [HEX] | 535 | 556 | 73,000 | 681.2 | x | - | - |
Alexa Fluor 555 [Alexa555] | 555 | 565 | 1,50,000 | 940 | x | x | x |
Bodipy TMR-X [BOTMRX] | 544 | 570 | 56,000 | 610.55 | x | x | x |
Cyanine3 [CY3] | 552 | 570 | 1,50,000 | 444.6 | x | - | - |
ATTO 550 [ATTO550] | 554 | 576 | 1,20,000 | 691 | x | x | x |
TAMRA [TAM] | 544 | 576 | 90,000 | 512.50 / 612.70 | x | x | - |
Rhodamine Red [RRE] | 560 | 580 | 1,29,000 | 770 | x | x | x |
ATTO 565 [ATTO565] | 563 | 592 | 1,20,000 | 608 | x | x | x |
ROX [ROX] | 575 | 602 | 82,000 | 632.78 | x | x | x |
Texas Red [TxRed] | 583 | 603 | 1,16,000 | 818 | x | x | x |
Cyanine3.5 [CY35] | 588 | 604 | 1,50,000 | 544.7 | x | - | - |
LightCycler 610 [LC610] | 590 | 610 | n.a. | 761.48 | x | x | x |
ATTO 594 [ATTO594] | 601 | 627 | 1,20,000 | 903 | x | x | x |
DY-480 XL [DY480] | 500 | 630 | 50,000 | 612.68 | x | x | x |
DY-610 [DY610] | 610 | 630 | 80,000 | 743 | x | x | x |
ATTO 610 [ATTO610] | 615 | 634 | 1,50,000 | 488 | x | x | x |
LightCycler 640 [LC640] | 625 | 640 | n.a. | 824.68 | x | x | x |
Bodipy 630/650 [BO630] | 625 | 640 | 1,01,000 | 662.6 | x | x | x |
ATTO 633 [ATTO633] | 629 | 657 | 1,30,000 | 649 | x | x | x |
Alexa Fluor 647 [Alexa647] | 650 | 665 | 2,39,000 | 957 | x | x | x |
Bodipy 650/665 [BO650] | 650 | 665 | 1,01,000 | 645.6 | x | x | x |
ATTO 647N [ATTO647N] | 644 | 669 | 1,50,000 | 743 | x | x | x |
Cyanine5 [CY5] | 649 | 670 | 2,50,000 | 470.63 | x | - | - |
DY-649 [DY649] | 655 | 674 | 2,50,000 | 942 | x | x | x |
Cyanine5.5 [CY55] | 675 | 694 | 2,50,000 | 570.74 | x | - | - |
ATTO 680 [ATTO680] | 680 | 700 | 1,25,000 | 623 | x | x | x |
DY-682 [DY682] | 690 | 709 | 1,40,000 | 906 | x | x | x |
ATTO 700 [ATTO700] | 700 | 719 | 1,20,000 | 663 | x | x | x |
ATTO 740 [ATTO740] | 740 | 764 | 1,20,000 | 565 | x | x | x |
DY-782 [DY782] | 782 | 800 | 1,70,000 | 932 | x | x | x |
Product specifications: | |||||||
Synthesis scales from 0.05 to 1.0 umol | |||||||
Purification options: HPSF, HPLC | |||||||
Sequence lengths: 5-80 bases | |||||||
TAT: 3-4 weeks | |||||||
Purification option | Synthesis scale1 [umol] | 0.05 | 0.2 | 1 | |||
HPLC | Minimum yield [OD]2 | 2 | 3 | 6 |