Hairpin shaped hybridization probes with an internally quenched fluorophore to detect specific sequences.
Molecular Beacons are comparable with our Dual Labeled Probes. They are also available in a multitude of fluorescent dyes and quencher options. Molecular beacons, or molecular beacon probes known hybridization probes that can quenched the presence of specific nucleic acids in homogenous solutions. Beacons are hairpin like structure with an internally fluorescent dyes which act and bind to a target nucleic acid sequence.
Various dyes combination for synthesis
5'
Reporter
Abs [nm]
Em [nm]
3'
Quencher
FAM
495
520
TAM, BHQ1, DAB, Eclip
TET
521
536
DAB
JOE
520
548
TAM
HEX
535
556
BHQ1, DAB
Cyanine3
552
570
BHQ2, DAB
ATTO 550
554
576
DAB
TAMRA
544
576
BHQ2
ROX
575
602
BHQ2
Texas Red
583
603
BHQ2
Cyanine5
649
670
BHQ2, BBQ650
Product specifications:
. HPLC purification is included
. Wobbles (non-defined ratio) possible
. TAT of 3-4 weeks
. Delivery: lyophilised or concentration adjusted in tubes
. Quality control by OD measurement and MALDI-TOF MS
