Chimeric oligos with RNA, DNA, PTO and 2'O-Methyl-RNA

The use of 2'O-Methyl-RNA in chimeric antisense oligos increases nuclease stability of the oligo.

Chimeric oligonucleotides consist of mixed DNA and RNA bases including methylated bases or phosphorthioate bounds (PTO) in various numbers and compositions.
O-Methyl-RNA / chimerics are delivered in 2ml screw cap tubes according to your specification:

* Hugh variety of RNA modifications

* HPLC purified

* Synthesis scales from 0.05 umol up to 1.0 umol

* Sequence lengths from 6 to 80 bases

* Quality control by OD measurement and MALDI-TOF MS

* Delivery occurs lyophilised in tube format

Product specifications

* HPLC purified

* Synthesis scales from 0.05 umol up to 1.0 umol

* Minimum yields in OD

* Sequence lengths: 6 - 80 bases

* Quality control by OD measurement and MALDI-TOF

* Turnaround time: 3-4 weeks

* Delivery: lyophilised in 2ml screw cap tubes

HPLC is required for purification of chimeric oligos. We recommend to perform a Na+ salt exchange with commercially available desalting columns (e.g. NAP or MicroSpin columns) before use in cells or live animals.

To differentiate between 2'O-Methyl-RNA, PTO, RNA and DNA bases enter your sequence as follows on the order page:

* DNA: ACGT
* RNA: (ACGU)
* PTO: A*C*(G*U)
* 2'-O-Methyl-RNA: [2OMeA], [2OMeC], [2OMeG], [2OMeU]
The synthesis scale indicates the initial amount of 3'-bases

PTOs, the defense line against nucleases

Phosphorothioate (PTOs) are the most widely used nuclease resistant oligos for antisense applications.

In PTO oligos, a non-bridging oxygen is replaced by a sulfur atom. Therefore, PTOs are also known as "S-oligos". Phosphorothioate bounds can be introduced to an oligo

* at the 5'- or 3'-end
--> Inhibits exonuclease degradation
* Internally
--> Limit the attack by endonucleases

PTO oligos can show greater non-specific protein binding than unmodified phosphodiester (PO) oligos. They can lead to toxic effects or cause artefacts building when present in high concentrations.
These problems can be reduced or eliminated by using chimeric designs, which limit the number of phosphorothioate internucleoside linkages within the oligo

PTO specifications

* Synthesis scales from 0.01 to 10 umol
* Purification options: HPSF, HPLC, PAGE
* Oligo lengths: 5-120 bases
* TAT: 3-4 weeks

Purification option	Synthesis scale1 [umol]	0.01	0.05	0.2	1
HPSF	Minimum yield [OD]2	2	4	8	20
HPLC	Minimum yield [OD]2	1	3	5	15
PAGE	Minimum yield [OD]2	-	0.5	0.5	-

1. The synthesis scale indicates the initial amount of 3'-bases.
2. There is no OD guarantee for oligos <18 and >35 bases or for oligos with multiple modifications.