Select your RNA modification according to their possible labelling positions:
Fluorescent
dyes
5'
Int
3'
FAM [FAM]
x
-
-
Fluorescein
[FLU]
-
-
x
Fluorescein-dT
[FLUdT]
-
x
-
TET [TET]
x
-
-
HEX [HEX]
x
-
-
Cyanine3
[CY3]
x
-
-
TAMRA [TAM]
x
-
x
TAMRA-dT
[TAMdT]
-
x
-
Cyanine5
[CY5]
x
-
-
Non fluorescent modifications
5'
Int
3'
Amino-C6
[AmC6]
x
-
-
Amino-C12
[AmC12]
x
-
-
Amino-C3/C6/C7
[AmC3] [AmC6] [AmC7]
-
-
x
Biotin [BIO]
x
-
x
Biotin-TEG
[BIOTEG]
x
-
x
Cholesterol
[CHOL]
-
-
x
Cholesteryl-TEG
[CholTEG]
x
-
-
Phosphate
[PHOS]
x
-
x
Spacer, linker, base & sugar modifications
5'
Int
3'
2'-Deoxyinosine
[I]
-
x
x
2'-Deoxyuridine
[U]
-
x
x
2',3'
dideoxyC [23ddC]
-
-
x
5-Methyl-dC
[5MedC]
-
x
x
Amino-C6-dT
[AmC6dT]
-
x
-
Biotin-dT
[BIOdT]
-
x
-
dSpacer
[Spd]
-
x
-
Spacer C3
[SpC3]
-
x
x
Wobble [WOB]
(defined ratio)
-
x
-
Dark quencher
5'
Int
3'
Dabcyl [DAB]
-
-
x
Dabcyl-dT
[DABdT]
-
x
-
Eclipse
[Eclip]
-
-
x
BHQ1 [BHQ1]
-
-
x
BHQ1-dT
[BHQ1dT]
-
x
-
BHQ2 [BHQ2]
-
-
x
BBQ650
[BBQ650]
-
-
x
Product specifications:
* Synthesis scales from 0.05 to 1.0 umol
* HPLC purified
* Guaranteed purity of >80% measured by CGE
* Minimum yields in OD
* Quality control by OD measurement and MALDI-TOF
* Sequence lengths from 10 - 80 bases
* Turnaround time: 3-4 weeks
* Delivery lyophilised in tube format
Purification
option
Purity
Synthesis
scale [umol]
0.05
0.2
1
HPLC
>80%
Minimum Yield [OD]
0.5
2
2.5
The synthesis scale indicates the initial amount of 3'-bases.
