Spacer, linkers, base & sugar modifications and their structures
These oligos are characterised by
guaranteed yields
We guarantee minimum yields in OD
maximum flexibility
Benefit from HPLC purification options in 3 different synthesis scales.
highest quality
OD measurement and MALDI-TOF MS ensure highest demands

--> 2'-Deoxyinosine [I]

2'-Deoxyinosine (INO) is a universal base that is less destabilising than mismatches found within a wobble or degenerated base.

--> 2'-Deoxyuridine [U]

2'-Deoxyuridine (URI) are often used as a substitute for dT.

--> 5-Methyl-dC [5MedC]

5-Methyl-dC stabilises the DNA duplex due to an increase in the melting temperature during the replacement of the dC.

--> Amino-C6-dT [AmC6dT]

Amino C6-dT allows internal modifications within the oligonucleotides sequence.

--> Biotin-dT [BIOdT]

Biotin-dT can be used for an internal biotin modification (e.g. for hybridisation studies).

--> 2',3'-dideoxyC [23ddC]

3'-ddC prevents extension by DNA polymerases and prevents degradation by 3'-exonucleases.

--> dSpacer [Spd]

The dSpacer is used to create a stable abasic site within an oligonucleotide.

--> Spacer C3 [SpC3]

Spacer C3 can be coupled to the 3'-end and internally of an oligonucleotide sequence. This can prevent e.g. the elongation of the oligo during a PCR without a major influence to its annealing properties. Oligos with C3 spacers are therefore ideal hybridisation probes in PCR reactions.

--> Wobble [WOB] (defined ratio)

The following IUB code is used to describe degenerated bases (wobbles). Wobbles are available with a defined and non defined ratio.

Product specifications:

--> Synthesis scales from 0.05 to 1.0 µmol
--> Purification options: HPLC
--> Sequence lengths: 5-80 bases
--> TAT: 2-3 weeks

1 The synthesis scale indicates the initial amount of 3'-bases
2 There is no OD guarantee for oligos <18 and >35 bases or for oligos with multiple modifications.